Protection of cell
proteins against free-radical attack by nootropic drugs: scavenger effect of pyritinol confirmed
by electron spin resonance spectroscopy.
Pavlik A, Pilar J.
Institute of Physiology,
Czechoslovak Academy of Sciences, Prague
Neuropharmacology 1989 Jun;28(6):557-61
The potency of nootropic drugs to protect cell proteins and lipids against
free radical attack was studied. In an in vitro system, generating hydroxyl
radicals by a Fenton-type reaction, the soluble proteins (bovine serum albumin
and cytosol protein from brain) were quickly insolubilized and precipitated.
Pyritinol (and tamitinol) exhibited the best protection against
insolubilization of protein while centrophenoxine (meclophenoxate) and its
dimethylaminoethanol moiety were less effective, piracetam (and oxiracetam)
being without effect. The lipid peroxidation induced by free radicals from
cyclic redox reactions of iron-ascorbate was not influenced by pyritinol,
indicating the selectivity of its scavenger action. The efficient scavenging
of hydroxyl radicals by pyritinol was confirmed by electron spin resonance
spectroscopy measurement of hydroxyl radicals entrapped by spin trap.
Millimolar concentrations of pyritinol competitively decreased the formation
of spin adducts. The results suggest that the protective effect of pyritinol
against free-radical induced derangement of cell proteins may be an important
part of its antirheumatic, as well as nootropic, action.